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1. Which double-stranded DNA molecule has the highest melting temperature?(Required)

An oligonucleotide with a repeating sequence of A-A-A at the 5’ end

A molecule of 5,000 base pairs with a high number of A-T base pairs

An oligonucleotide with a large number of repeating C-G-C codons

A DNA polymer with a high number of A-T-T trinucleotide repeats

2. Which base pair sequence is most likely to serve as a binding site for a restriction endonuclease?(Required)

A-T-T-C-A T-A-A-G-T

C-T-A-C-T-G G-A-T-G-A-C

C-A-C G-T-G

A-A-G-C-T-T T-T-C-G-A-A

3. Cloning a human gene into a bacterium to make a large molecular probe requires which vector?(Required)

Plasmid

Bacterial microsome

30S bacterial ribosome

Single-stranded DNA

4. What process can be used to make a DNA probe produce a fluorescent or chemiluminescent signal?(Required)

Enzymatic attachment of acridinium esters to terminal ends of the probe

Substitution of biotinylated or fluorescent nucleotides into the probe

Splicing the gene for β-galactosidase into the probe

Heat denaturation of the probe followed by acid treatment

5. What term describes the products produced when DNA is digested by restriction endonucleases?(Required)

Mosaicisms

Chimeras

Amplicons

Restriction fragment length polymorphisms

6. What reagent is most commonly used to stain DNA separated by electrophoresis?(Required)

Silver nitrate

Nicotinamide adenine dinucleotide

Cationic dye

Ethidium bromide

7. Which technique is used to detect DNA containing a specific base sequence by applying a labeled probe to DNA bands immobilized onto nitrocellulose paper after electrophoresis?(Required)

Southern blot

Northern blot

Dot blot

Western blot

8. Which of the following types of mutation causes the premature termination of protein synthesis?(Required)

Missense

Insertion

Frame shift

9. In humans, which component of a gene is translated into a protein?(Required)

Intron

Exon

Promoter

TATA box

10. Which statement best describes a DNA polymorphism?(Required)

A point mutation arising in a gene

Any change in DNA that is associated with abnormal function

A change in the base sequence of DNA that is translated into an abnormal protein

A variation in DNA that occurs with a frequency of at least 1%

Tutorial Course for the preparation of the Categorial certification exam in Molecular Biology (MB (ASCPi)

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