ASCP Mock

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1. This refers to a means by which large quantities of DNA can be amplified from a small amount of DNA template?(Required)

PCR

DNA mass production

DNA synthesis

DNA birth

2. With regards to PCR, which of the following is NOT accurate?(Required)

Denature strands at 94 degrees

Base pairing of primers at 60 degrees

Elongation of primers using Taq polymerase at 72 degrees

Filtration of DNA at 40 degrees

3. Which of the following PCR techniques is the best one for detecting point mutation in DNA?(Required)

RT PCR

PCR

Allele specific PCR

Anchored PCR

4. What happens after many cycles of PCR?(Required)

Exponential amplification

Diminished amplification

Product lysing

DNA translation

5. Which of the following is NOT true about the Taq DNA polymerase?(Required)

It allows for the ability to use PCR to automate the process of amplification

It can be heat resistant

It is ubiquitous in nature except for the hot springs

It is most efficient at a certain optimal temperature

6. The PCR technique used to specifically amplify only one strand of the target DNA by using unequal primer concentration is:(Required)

qPCR

Asymmetric PCR

Anchored PCR

Nested PCR

7. Sarah wants to turn up the heat on her heat block to thaw her DNA samples at over 100 degrees for an hour. What is the likely result of her PCR?(Required)

She will get much more amplified DNA product

She will not get a significant amplified DNA product

She will get only minimal synthesis

She will likely not get any synthesis

8. When setting up her thermocycler, Sarah wants to anneal her DNA strands at a temperature much higher than 60 degrees. What is the likely result of her action?(Required)

The DNA strands will likely not anneal as desired

The DNA strands will likely anneal more optimally

The DNA strands will anneal, and then form a triple helix

The DNA strands will anneal but will not form a double helix

9. The PCR technique widely used in bacterial genomic studies for screening of plasmid inserts is:(Required)

Anchored PCR

Colony PCR

qPCR

pPCR

10. When setting up her thermocycler, Sarah wants to base pair her DNA strands at a temperature much lower than 60 degrees. What is the likely result of her action?(Required)

She will only get single stranded DNA products

The primers will likely stick to other regions of the genome

The primers will stick to the 5′ ends only

The primers will stick to the 3′ ends only

11. Which of the following is true?(Required)

Elongation occurs at 90 degrees

Taq polymerase activity is near optimal at 72 degrees

Taq polymerase activity is near optimal at 50 degrees

Polymerase is not required for the elongation step of PCR

12. This PCR variant uses degenerate primers to amplify unknown sequences of DNA, related to a known DNA sequence is:(Required)

Degenerate PCR

Multiplex PCR

Hotstart PCR

Inverse PCR

13. TWhich of the following is true about agarose gel electrophoresis?(Required)

Used to separate bad proteins from good ones

Used to separate different DNA molecules based on their length

Used to lyse proteins with ethidium bromide (EtBr) staining

Used to identify globular proteins

14. Which of the following is true about ethidium bromide?(Required)

Intercalates underneath stacked bases of DNA double helix

Intercalates between the stacked bases of the DNA double helix

Facilitates the delivery of mutation into DNA strands

Facilitates the stacking of bases of DNA double helix

15. If the sequence of only one strand is known for primer synthesis, the best PCR variant that allows amplification of DNA with only one known sequence is:(Required)

Hotstart PCR

Inverse PCR

Multiplex PCR

Nested PCR

16. True or false: Short strands of DNA usually migrate faster through gel than long ones(Required)

True

False

17. Which of the following is NOT true about reverse transcription PCR (RT-PCR)?(Required)

Can be used to detect RNA

Used to convert mRNA to cDNA

Retroviruses use reverse transcriptase to convert ssRNA to dsDNA

Coronaviruses use reverse transcriptase to convert ssDNA to ssRNA

18. The most efficient PCR technique that uses primer mix to detect deletions or duplications in a large gene is:(Required)

Anchored PCR

Nested PCR

Miniprimer PCR

Multiplex PCR

19. Which of the following is NOT true about quantitative real-time PCR (qRT-PCR)?(Required)

It uses a fluorescent dye to quantify the abundance of transcripts

It uses an instrument to detect fluorescence of probed targets

Quantitative in nature

It shows only the plateaued number of copies of DNA made every 30 minutes

20. Which of the following is NOT an advantage of PCR?(Required)

Requires large amounts of template DNA

Very sensitive

Generates multiple copies of target DNA

Usually takes only a few hours to complete

21. The PCR technique designed to minimize amplification of non-specific PCR products by using 2 primer sets is:(Required)

Multiplex PCR

Mini primer PCR

Nested PCR

qPCR

22. The PCR technique designed to minimize amplification of non-specific PCR products by using 2 primer sets is:(Required)

It can be sensitive to temperature changes

It requires DNA sequence information that may be difficult to obtain

Only large fragments are amplified

There can be a high error rate

23. Which of the following is NOT an application of PCR?(Required)

Genomic cloning

Identification of psychological ailments

DNA fingerprinting

24. The PCR variant designed for the study of RNA expression level is:(Required)

Reverse transcriptase or RT PCR

qPCR

Anchored PCR

Traditional PCR

25. In which of these cases does genetic testing NOT apply?(Required)

Diagnosis of genetic disorders

Prenatal diagnosis

Newborn screening and carrier detection

Identifying the main causes of environmental pollution

26. Which of the following does NOT apply to DNA fingerprinting / Short Tandem Repeats (STR)?(Required)

A short region of the genome

Highly polymorphic and can detect differences or markers in population

Not detected by PCR

Used in forensics and paternity testing